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Image Search Results
Journal:
Article Title: Enamel Protein Regulation and Dental and Periodontal Physiopathology in Msx2 Mutant Mice
doi: 10.2353/ajpath.2010.091224
Figure Lengend Snippet: Immunolocalization of β-galactosidase protein in Msx2 knock-in heterozygous mutant mouse molar crown. MSX2 expression is shown labeled in brown at 1 (A, D, H) and 2 (B, C, I) postnatal weeks. A represents a sagittal view of a molar, with the cervical area on the left and the occlusal part on the right. Black rectangles are enlarged in E–H. Ameloblast stage was determined according to their morphology and location in the molar crown. At 1 week, most ameloblasts are at the secretion stage while at 2 weeks, most of the ameloblasts are at the maturation stage. Ab PS, presecretion stage ameloblasts; Ab S, secretion stage ameloblasts in 3 areas: Ab S1 corresponds to the cervical part of the crown where enamel thickness is reduced, Ab S2 corresponds to the middle part of the crown where enamel thickness is greatest, and Ab S3 corresponds to the cusp area where enamel thickness decreases. Ab EF, ameloblast facing the enamel-free area; Ab M, maturation stage ameloblasts; IEE, inner enamel epithelium; SR, stellate reticulum; SI, stratum intermedium. Strong labeling is noted in presecretion stage ameloblasts (D), maturation stage ameloblasts (I) and in ameloblasts facing the enamel-free area in the occlusal crown (H). In secretion stage ameloblasts, labeling decreases with enamel thickness: at the middle region of the crown, corresponding to the thickest enamel layer, brown labeling is less intense (Ab S2, F); at the cervical and occlusal parts of the crown where the enamel layer is thinner, labeling is stronger (Ab S1 and Ab S3, E–G). Experiments without primary antibody are controls (B and C).
Article Snippet: Specimens were incubated for 1 hour at room temperature in a humidified chamber with a
Techniques: Knock-In, Mutagenesis, Expressing, Labeling
Journal:
Article Title: Enamel Protein Regulation and Dental and Periodontal Physiopathology in Msx2 Mutant Mice
doi: 10.2353/ajpath.2010.091224
Figure Lengend Snippet: Immunolocalization of β-galactosidase protein in Msx2 knock-in heterozygous mouse root. Epithelial life cycle is followed in an incisor (A) and first molar (B–E). Different cells are recognized according to their morphology and position. At postnatal week 2, MSX2 expression is evident in odontoblasts, pulp cells, ERM (arrows), and Hertwig epithelial root sheath (between the vertical black lines). HERS, Hertwig epithelial root sheath). Black rectangles in B are shown enlarged in D and E.
Article Snippet: Specimens were incubated for 1 hour at room temperature in a humidified chamber with a
Techniques: Knock-In, Expressing